. 2014 Apr 3;5:53. doi: 10.3389/fphar.2014.00053

Table 4.

Ions and molecules tested as gating pore blockers.

Channel	Mutation	Location	Ions		Guanidinium derivatives		References
			Block	No effect	Block	No effect
dShaker	R362C/E283D	R1/E1	Mg²⁺		Ethyl-guanidium		Tombola et al., 2005
rNa_v1.4	R666G	DII/R2	Ca²⁺, Zn²⁺, Ba²⁺				Sokolov et al., 2007
rNa_v1.4	R663H	DII/R1		Li⁺, Ni²⁺, Cd²⁺, Ba²⁺			Struyk and Cannon, 2007
rNa_v1.4	R666G	DII/R2		Ca²⁺, Zn²⁺, Ba²⁺			Struyk et al., 2008
N.at-K_v3.2	None^*	None^*		Ba²⁺			Klassen et al., 2008
rNa_v1.4	R666G	DII/R2	Ca²⁺, Zn²⁺, Y³⁺, Ba²⁺, La³⁺, Yb³⁺, Lu³⁺, Hf⁴⁺, Tl³⁺		1-(2,4-xylyl) guanidine-carbonate	Ethyl- guanidium	Sokolov et al., 2010
hH_v1	R211S	R3	Zn²⁺				Berger and Isacoff, 2011
rNa_v1.4	R1125Q	DIII/R2	Ni²⁺, Zn²⁺	Ca²⁺, Ba²⁺			Francis et al., 2011
hNa_v1.5	R219H	DI/R1		Ni²⁺, Zn²⁺, Cd²⁺,La³⁺		Ethyl- guanidium	Gosselin-Badaroudine et al., 2012b

For N.at-K_v3.2, no substitution was required to induce a gating pore.