Fig. 1.

D-Tailor enables multidimensional analysis and design of DNA sequences. D-Tailor provides a flexible and extendable architecture to interrogate different sequence properties (box in the middle). The left panel depicts an example of the retrieval process of two properties (RNA structure and motif prediction) from multiple input sequences that can come from either FASTA or CSV files. The resulting score profile can be used to identify general trends and further define ideal parameter ranges for the design objectives. The right panel shows the design mode of D-Tailor, wherein a seed sequence is evolved to meet a user-defined combination of sequence properties. The figure depicts a full-factorial design for two different properties of interest (RNA structure and motif scores) with three levels each (low, medium and high), which yields a total of nine different combinations (colored areas).