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. 2014 Mar 4;3(3):e150. doi: 10.1038/mtna.2014.3

Figure 3.

Figure 3

Specific downregulation of EGFR T790M expression and downstream signaling by DzT. (a) RT-qPCR analysis of EGFR mRNA in cells treated with DzT (n = 3). Cells were harvested 48 hours after transfection with DzC or DzT (100 nmol/l). The relative amount of EGFR mRNA was normalized to ACTB mRNA. The data are presented as means ± SD and were analyzed by Student's t-test. Asterisks denote statistical significant differences (P ≤ 0.005). (b) Immunoblot analysis of EGFR and its downstream signaling pathways. Cells were harvested 72 hours after transfecting with 100 nmol/l DzC or DzT. EGFR in wild-type cells was activated by adding 100 ng/ml EGF 15 minutes before cell lysates were harvested. EGFR, epidermal growth factor receptor; mRNA, messenger RNA; RT-qPCR, quantitative reverse transcription polymerase chain reaction.