Skip to main content
. Author manuscript; available in PMC: 2015 Mar 7.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Feb 12;445(2):445–450. doi: 10.1016/j.bbrc.2014.02.015

Figure 2.

Figure 2

a) SPR sensorgram for the immobilization of EctopoI via capture covalent method. The arrows show the start of immobilization and buffer wash. b) Representative SPR signals showing stability of sensor surface; with buffer before enzyme immobilization and after capture covalent immobilization of the enzymes. c) Representative SPR profile for enzyme-pBAD/Thio interaction: 1 to 2 association, 2 to 3 saturation, 3 to 4 minor signal shift due to manual changing of valve, 4 to 5 dissociation, and 5 to 6 regeneration.