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. Author manuscript; available in PMC: 2015 Mar 7.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Feb 12;445(2):445–450. doi: 10.1016/j.bbrc.2014.02.015

Figure 3.

Figure 3

SPR sensorgrams for a) pBAD/Thio binding to EctopoI, b) for pBAD/Thio binding to Mg2+ bound EctopoI and c) for pBAD/Thio binding to MttopoI. d) Plot of equilibrium SPR response vs pBAD/Thio concentration. The symbols are average experimental data of three different measurements with error bars as standard deviation and the continuous lines are the simple hyperbolic fit (explained in text).