Table 1.
Quantitative values (normalized weighted spectra) for the identified extracellular proteins (n = 4 per group, values are mean ± SEM).
| Identified Proteins | Molecular weight | Young WT | Young KO | Middle WT | Middle KO | Old WT | Old KO | Accession number |
|---|---|---|---|---|---|---|---|---|
| Secreted proteins | ||||||||
| Annexin A2 | 39 kDa | 0 | 0 | 0 | 0 | 0.5 ± 0.5 | 0.5 ± 0.5 | gi∣12849385 |
| Apolipoprotein O | 21 kDa | 0 | 0.8 ± 1.0 | 0 | 0 | 0 | 0 | gi∣123122452 |
| Collagen type I alpha-1 | 138 kDa | 10.0 ± 0.4 | 7.8 ± 2.6 | 12.5 ± 2.6 | 7.0 ± 0.8 | 10.5 ± 1.2 | 6.0 ± 1.8 | gi∣34328108 |
| Collagen type I alpha-2 | 130 kDa | 6.0 ± 0.7 | 3.5 ± 1.3 | 9.5 ± 1.6 | 3.5 ± 1.3 | 9.3 ± 1.4 | 7.0 ± 0.6 | gi∣111120329 |
| Collagen type III alpha-1 | 139 kDa | 0.3 ± 0 | 0.3 ± 0 | 1.5 ± 0.8 | 0.5 ± 0 | 1.3 ± 0 | 0.8 ± 0 | gi∣74184771 |
| Collagen type IV alpha-2 | 167 kDa | 1.5 ± 0.6 | 0.8 ± 0.6 | 3.8 ± 0.6 | 2.3 ± 0.6 | 4.3 ± 0.8 | 3.0 ± 0 | gi∣556299 |
| Collagen type VI alpha-1 | 108 kDa | 7.8 ± 0.6 | 8.8 ± 0.5 | 7.5 ± 1.2 | 7.8 ± 1.9 | 6.0 ± 1.1 | 6.3 ± 0.6 | gi∣6753484 |
| Collagen type VI alpha-2 | 110 kDa | 4.0 ± 0.9 | 6.0 ± 1.2 | 7.8 ± 1.5 | 9.5 ± 1.4 | 11.0 ± 0.7 | 9.5 ± 1.7 | gi∣22203747 |
| Collagen type VI alpha-3 | 287 kDa | 41.8 ± 6 | 46.3 ± 7 | 45.0 ± 7 | 51.5 ± 7 | 51.3 ± 5 | 48.0 ± 4 | gi∣148708135 |
| Collagen type XV | 140 kDa | 0 | 0 | 0 | 0.3 ± 0.3 | 0 | 0 | gi∣11037306 |
| Collagen type XXIV alpha-1 | 176 kDa | 0 | 0 | 0.3 ± 0.3 | 0 | 0 | 0 | gi∣116326001 |
| Fibrillin-1 | 312 kDa | 0 | 0.3 ± 0.3 | 0 | 0 | 0 | 0.5 ± 0.5 | gi∣118197277 |
| Fibrinogen, alpha | 87 kDa | 0.3 ± 0 | 0 | 0 | 1.0 ± 0.6 | 3.3 ± 1.7 | 3.0 ± 1.3 | gi∣148683476 |
| Fibrinogen, beta | 55 kDa | 0.0 | 0 | 0.3 ± 0 | 0.5 ± 0.5 | 1.3 ± 0.9 | 0.5 ± 0.5 | gi∣33859809 |
| Fibrinogen, gamma | 49 kDa | 0.0 | 0 | 0 | 0 | 0.5 ± 0.5 | 0 | gi∣148683478 |
| Laminin alpha-2 | 344 kDa | 0.8 ± 0 | 1.5 ± 0.7 | 0.5 ± 0.5 | 0.3 ± 0.3 | 0 | 0.3 ± 0.3 | gi∣117647249 |
| Laminin beta-1 | 204 kDa | 1.0 ± 0.7 | 0.5 ± 0.3 | 0.3 ± 0.3 | 0.3 ± 0.3 | 1.0 ± 0.6 | 0.3 ± 0.3 | gi∣148704971 |
| Laminin beta-2 | 197 kDa | 0.3 ± 0.3 | 1.5 ± 0.9 | 0.5 ± 0.5 | 1.5 ± 0.6 | 0.5 ± 0.3 | 1.3 ± 0.5 | gi∣31982223 |
| Laminin gamma-1 | 179 kDa | 1.8 ± 0.5 | 1.0 ± 0.4 | 2.0 ± 0.8 | 5.5 ± 1.2 | 2.3 ± 0.9 | 2.5 ± 0.6 | gi∣148707495 |
| Nidogen-1 | 137 kDa | 1.0 ± 0.6 | 1.3 ± 0.5 | 0 | 0.3 ± 0.3 | 0.5 ± 0.5 | 0.8 ± 0.3 | gi∣171543883 |
| Perlecan | 470 kDa | 4.8 ± 0.5 | 4.8 ± 0.3 | 3.5 ± 1.0 | 5.5 ± 1.2 | 5.8 ± 0.8 | 6.8 ± 1.3 | gi∣183979966 |
| Prelamin-A/C | 74 kDa | 0.3 ± 0 | 0.8 ± 0.5 | 0.3 ± 0 | 0 | 0 | 0 | gi∣162287370 |
| Serum albumin | 69 kDa | 0 | 0 | 0 | 1.0 ± 0.5 | 0.3 ± 0.3 | 0 | gi∣163310765 |
| Troponin I, cardiac muscle | 24 kDa | 15.8 ± 0.9 | 13.5 ± 1.2 | 11.8 ± 2.1 | 14.3 ± 0.9 | 10.8 ± 2.6 | 9.8 ± 2.0 | gi∣6678393 |
| Troponin T2, cardiac | 32 kDa | 0.8 ± 0.5 | 1.0 ± 0.7 | 0.5 ± 0.5 | 0 | 0 | 0 | gi∣148707615 |
| Von Willebrand factor A domain-containing protein 8 | 213 kDa | 2.3 ± 0.6 | 3.5 ± 0.6 | 1.8 ± 0.4 | 2.0 ± 0.5 | 3.0 ± 0.5 | 3.0 ± 0.8 | gi∣226958579 |
|
| ||||||||
| Cell membrane proteins | ||||||||
| Alpha-sarcoglycan | 43 kDa | 0 | 0 | 0 | 0 | 0 | 0.3 ± 0.3 | gi∣2411510 |
| Aminopeptidase | 103 kDa | 0 | 0.5 ± 0.5 | 0.5 ± 0.5 | 1.5 ± 1.0 | 0 | 0.5 ± 0.5 | gi∣1184161 |
| ATP1a1 protein | 108 kDa | 5.3 ± 1.9 | 6.8 ± 2.9 | 4.3 ± 3.0 | 6.5 ± 3.8 | 2.0 ± 0.8 | 1.3 ± 0.8 | gi∣16307541 |
| ATP-binding cassette (ALD) 3 | 75 kDa | 0 | 0 | 0 | 0 | 0 | 0 | gi∣14318642 |
| ATP-binding cassette (MDR/TAP) 8 | 78 kDa | 0.8 ± 0.5 | 0.0 | 0.5 ± 0.5 | 0 | 0 | 0 | gi∣148671187 |
| EH domain-containing protein 4 | 61 kDa | 2.0 ± 0.7 | 1.5 ± 0.9 | 2.8 ± 0.9 | 4.0 ± 0.4 | 1.5 ± 0.5 | 2.8 ± 0.3 | gi∣31981592 |
| Guanine nucleotide-binding protein, subunit beta-2 | 37 kDa | 0 | 1.3 ± 0.6 | 0.5 ± 0.2 | 0.5 ± 0.3 | 0.5 ± 0.3 | 0 | gi∣13937391 |
| Guanosine diphosphate dissociation inhibitor 2 | 53 kDa | 0 | 0 | 0 | 1.0 ± 1 | 0 | 0 | gi∣148700276 |
| Neutral cholesterol ester hydrolase 1 | 46 kDa | 4.5 ± 0.3 | 3.0 ± 0.4 | 0 | 0.3 ± 0.3 | 0 | 0.5 ± 0.5 | gi∣30520239 |
| PDZ and LIM domain protein 5 | 24 kDa | 2.5 ± 0.5 | 3.3 ± 0.6 | 1.5 ± 0.6 | 2.0 ± 0.4 | 0.5 ± 0.3 | 0.8 ± 0.8 | gi∣300069034 |
| Perilipin-4 | 139 kDa | 3.5 ± 1.2 | 5.0 ± 0.8 | 4.8 ± 0.6 | 6.0 ± 2.0 | 8.0 ± 2.0 | 6.8 ± 1.6 | gi∣157041252 |
| Peroxisomal membrane protein 20 | 17 kDa | 2.3 ± 0.3 | 3.5 ± 0.5 | 1.3 ± 1.3 | 1.5 ± 0.3 | 1.5 ± 0.5 | 1.5 ± 0.9 | gi∣6746357 |
| Platelet glycoprotein 4 | 53 kDa | 0 | 1.3 ± 1.3 | 0.5 ± 0.5 | 0 | 0 | 0 | gi∣74151899 |
| Protease, serine 15 | 109 kDa | 0.5 ± 0.5 | 0 | 0 | 0 | 0 | 0 | gi∣148706233 |
| Sodium/calcium exchanger 1 | 108 kDa | 0.5 ± 0.5 | 0.8 ± 0.8 | 0 | 0 | 0 | 0 | gi∣119120890 |
| Sorbin and SH3 domain-containing protein 1 | 83 kDa | 3.5 ± 1.6 | 4.0 ± 0.9 | 6.3 ± 0.6 | 5.0 ± 0.9 | 6.5 ± 0.9 | 5.3 ± 1.3 | gi∣78000154 |
| Transglutaminase | 77 kDa | 0 | 0 | 0 | 0 | 0 | 0.3 ± 0.3 | gi∣201941 |
| Tripartite motif-containing protein 72 | 53 kDa | 6.5 ± 1.3 | 8.0 ± 0.8 | 10.0 ± 0.7 | 10.3 ± 1.8 | 7.8 ± 1.3 | 11.3 ± 1.1 | gi∣121247302 |