Table 2.

Primers used in this study

Primer	Sequence	Analysis
cnaB F1	Forward 5′-TGTCATCAGCCATGGACGCATTAG-3′	Inactivation of cnaB
cnaB R1	Reverse 5′-TCCAGTTTCTGCAGCTATCCAGCC-3′
cnaB F2	Forward 5′-GGCTGGATAGCTGCAGAAACTGGA-3′
cnaB R2	Reverse 5′-GCCTCTATACATATGTTCGGTCGT-3′
cbpA F1	Forward 5′-CAGATGCTTACCATGGCACCTATGA-3′	Inactivation of cbpA
cbpA R1	Reverse 5′-CTTCAGTTGGCGGCCGCCTTTCAGTC-3′
cnaBcbpA F1	Forward 5′-CATAGACAACCATGGTCATGACAG-3′	Inactivation of cnaB and cbpA
cnaB R1	Reverse 5′-TCCAGTTTCTGCAGCTATCCAGCC-3′
cnaBcbpA F2	Forward 5′-GATCAGTCTCTGCAGCGAACTAGC-3′
cnaBcbpA R2	Reverse 5′-GATTGCATCTCATATGGTCGTTGTA-3′
UAcnaB F	Forward 5′-GACATAAGTCTAGAATTCCTC-3′	Expression of cnaB in UA159
UAcnaB R	Reverse 5′-GTCAGTTCTGTACATAAGACTTAAC-3′
UAcbpA F	Forward 5′-GAAAGCATCTCTAGAAAGTCTTAG-3′	Expression of cbpA in UA159
UAcbpA R	Reverse 5′-TAGCTTAGTGTACATTAACGCTG-3′
UAcnm F	Forward 5′-AGCGTTAATCTAGACTAACGTAAATC-3′	Expression of cnm in UA159
UAcnm R	Reverse 5′-CCTATTTTTAATGTACATCAGTTATG-3′
rCnmA F	Forward 5′-ACTAAGGCTCATATGAGTGATGTC-3′	Recombinant expression of CBD in E. coli
rCnmA R	Reverse 5′-TCCACACCGGATCCGGCATTAAC-3′
cnaB F3	Forward 5′-CTGGAATCATTCATTATCAA-3′	RT-PCR
cnaB R3	Reverse 5′-TCGGCATAACATTTC-3′
cnaBcbpA F3	Forward 5′-CGACAAGCGGAAAGCTG-3′	RT-PCR
cnaBcbpA R3	Reverse 5′-CTCAAACAGTGTCATTATAGA-3′
cbpA F3	Forward 5′-ACTGCTTTTCTGGGT-3′	RT-PCR
cbpA R3	Reverse 5′-CAGTACCAGTTCTAAAACC-3′
cbpAcnm F1	Forward 5′-CTTCAAGCCAGTCATCTGCT-3′	RT-PCR
cbpAcnm R1	Reverse 5′-GTTTTACTACCGTTGCCAAGG-3′
Cnm F1	Forward 5′-TGGAACCTTGCCATCA-3′	RT-PCR
Cnm R1	Reverse 5′-CGACCATTGAACCTTCGAC-3′

CBD, collagen-binding domain; E. coli, Escherichia coli; RT-PCR, reverse transcription–polymerase chain reaction. Restriction sites that were introduced for cloning purposes are underlined.