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. Author manuscript; available in PMC: 2014 Apr 10.
Published in final edited form as: Genesis. 2010 Oct 7;48(11):635–644. doi: 10.1002/dvg.20667

FIG. 3.

FIG. 3

Identification of the segregation of osteoblastic and chondrogenic lineages in osteo-chondroprogenitor cells using tamoxifen-inducible Cre recombinase. Sagittal section of the tibia harvested at E17.0 from Sox9Cre/+;R26R embryos and at E16.5 from Sox9CreERT2/+;R26R embryos injected with tamoxifen at E10.5 and E12.5. (a) Positive X-gal staining is detected in all chondrocytes of the growth plate and in the periosteum of Sox9Cre/+;R26R embryos. (b) Positive X-gal staining is detected in all chondrocytes of the growth plate and in the periosteum of Sox9CreERT2/+;R26R embryos injected with tamoxifen at E10.5. (c) Positive X-gal staining is detected in all chondrocytes of the growth plate, but not in the periosteum, of Sox9CreERT2/+;R26R embryos injected with tamoxifen at E12.5. Arrows indicate the periosteum.