Table 3.
The 17 proteins identified with higher confidence level (at least three unique peptide sequences matched) in this study. The P17, CD44, was only identified on the SF modified surface.
| Protein number | Swiss-Prot/ TrEMBL accession number |
Protein name | MW (Da) | Score | Match queries | PI | Sequence coverage | Match peptide |
|---|---|---|---|---|---|---|---|---|
| P01 | Q2M3C7 | A-kinase anchor protein SPHKAP | 186339 | 34 | 3 | 5.04 | 8% | R.EACAGEPEPFLSK.S + Carbamidomethyl (C); Phospho (ST) R.QSSMPDSRSPCSR.L + Carbamidomethyl (C); 4 Phospho (ST); Oxidation (M) R.SPVCHRQSSMPDSRSPCSR.L + Carbamidomethyl (C); Deamidated (NQ); 4 Phospho (ST); Oxidation (M) |
|
| ||||||||
| P02 | P05067 | Amyloid beta A4 protein | 86888 | 19 | 3 | 4.73 | 9% | K.WDSDPSGTKTCIDTK.E + 5 Phospho (ST) K.GAIIGLMVGGVVIATVIVITLVMLKK.K + Oxidation (M); Phospho (ST) R.ALEVPTDGNAGLLAEPQIAMFCGR.L + 2 Deamidated (NQ); Oxidation (M); Phospho (ST) |
|
| ||||||||
| P03 | Q9UKV3 | Apoptotic chromatin condensation inducer in the nucleus | 151771 | 19 | 3 | 6.08 | 2% | R.EREMER.R R.TSTSSSSVQAR.R + 7 Phospho (ST) K.QSADSSSSRSSSSSSSSSR.S + Deamidated (NQ); 9 Phospho (ST) |
|
| ||||||||
| P04 | Q9NR09 | Baculoviral IAP repeat-containing protein 6 | 529919 | 34 | 3 | 5.67 | 4% | R.SRGTPSGTQSSR.E + Deamidated (NQ); 3 Phospho (ST) R.TIPDKIGSTSGAEAANK.I + Deamidated (NQ) R.GRTIPDKIGSTSGAEAANK.I + Phospho (ST) |
|
| ||||||||
| P05 | P51685 | C-C chemokine receptor type 8 | 40817 | 18 | 3 | 8.66 | 4% | R.ESCEKSSSCQQHSSR.S + Carbamidomethyl (C); Deamidated (NQ); 3 Phospho (ST) R.ESCEKSSSCQQHSSR.S + Carbamidomethyl (C); 2 Deamidated (NQ); 5 Phospho (ST) R.ESCEKSSSCQQHSSR.S + Carbamidomethyl (C); 2 Deamidated (NQ); 5 Phospho (ST) |
|
| ||||||||
| P06 | Q9BV73 | Centrosome-associated protein CEP250 | 280967 | 19 | 3 | 5 | 5% | R.EPAQLLLLLAK.T K.GQLEVQIQTVTQAK.E + 4 Deamidated (NQ); Phospho (ST) K.AEHVRLSGSLLTCCLRLTVGAQSR.E R.SLFKRGPLLTALSAEAVASALHK.L + 3 Phospho (ST) |
|
| ||||||||
| P07 | O95067 | G2/mitotic-specific cyclin-B2 | 45253 | 18 | 3 | 9 | 12% | R.KKLQLVGITALLLASK.Y K.VPVQPTKTTNVNKQLKPTASVKPVQMEK.L + Deamidated (NQ); Oxidation (M); Phospho (ST) K.AQNTKVPVQPTKTTNVNK.Q + 3 Deamidated (NQ); 2 Phospho (ST) |
|
| ||||||||
| P08 | Q16478 | Glutamate receptor, ionotropic kainate 5 | 109195 | 42 | 4 | 8.54 | 7% | K.VSTIIIDANASISHLILRK.A + Deamidated (NQ); 2 Phospho (ST) R.LNCNLTQIGGLLDTK.G + 2 Deamidated (NQ); Phospho (ST) R.YQTYQRMWNYMQSK.Q + 4 Deamidated (NQ); Oxidation (M); 2 Phospho (ST); 2 Phospho (Y) R.LQYLRFASVSLYPSNEDVSLAVSRILK.S + 2 Deamidated (NQ) |
|
| ||||||||
| P09 | Q63HM2 | Pecanex-like protein C14orf135 | 132616 | 36 | 7 | 5.88 | 9% | K.GDLIKVLVWILVQYCSK.R K.GDLIKVLVWILVQYCSK.R + Deamidated (NQ) K.HQLKDLPGTNLFIPGSVESQR.V K.HQLKDLPGTNLFIPGSVESQR.V + Deamidated (NQ) K.HQLKDLPGTNLFIPGSVESQR.V + 2 Deamidated (NQ) R.LMWIMILECGYTYCSINIK.G + 2 Carbamidomethyl (C); Deamidated (NQ) K.KYVANTVFHSILAGLACGLGTWYLLPNR.I + Carbamidomethyl (C) |
|
| ||||||||
| P10 | O14497 | AT-rich interactive domain-containing protein 1A | 241892 | 27 | 8 | 6.24 | 6% | K.SKKSSSSTTTNEK.I + Deamidated (NQ); 6 Phospho (ST) K.HPGLLLILGKLILLHHK.H R.NSMTPNPGYQPSMNTSDMMGR.M + 2 Deamidated (NQ); Oxidation (M); 2 Phospho (ST) R.EMAVVLLANLAQGDSLAARAIAVQK.G + Deamidated (NQ); Oxidation (M) R.ITATMDDMLSTRSSTLTEDGAK.S + 2 Oxidation (M); 4 Phospho (ST) K.APGSDPFMSSGQGPNGGMGDPYSR.A + 4 Phospho (ST); Phospho (Y) R.GYMQRNPQMPQYSSPQPGSALSPR.Q + Oxidation (M); Phospho (ST) K.RNSMTPNPGYQPSMNTSDMMGR.M + Deamidated (NQ); Oxidation (M); 3 Phospho (ST); Phospho (Y) |
|
| ||||||||
| P11 | P50224 | Sulfotransferase 1A3/1A4 | 34174 | 23 | 4 | 5.68 | 6% | R.LIKSHLPLALLPQTLLDQK.V R.LIKSHLPLALLPQTLLDQK.V + Deamidated (NQ) R.LIKSHLPLALLPQTLLDQK.V + Deamidated (NQ) R.LIKSHLPLALLPQTLLDQK.V + 2 Deamidated (NQ) |
|
| ||||||||
| P12 | Q09666 | Neuroblast differentiation-associated protein AHNAK | 628699 | 39 | 3 | 5.8 | 2% | K.VHAPGLNLSGVGGKMQVGGDGVK.V + Deamidated (NQ); Oxidation (M); Phospho (ST) R.AGAISASGPELQGAGHSKLQVTMPGIKVGGSGVNVNAK.G + 2 Deamidated (NQ); Oxidation (M); 4 Phospho (ST) K.VKVPEVDVRGPK.V |
|
| ||||||||
| P13 | Q63HM2 | Pecanex-like protein C14orf135 | 132616 | 35 | 3 | 5.88 | 5% | R.TSCMPSSKMK.E + Carbamidomethyl (C); 2 Oxidation (M); 2 Phospho (ST) K.HQLKDLPGTNLFIPGSVESQR.V + Deamidated (NQ) K.HQLKDLPGTNLFIPGSVESQR.V + 2 Deamidated (NQ) |
|
| ||||||||
| P14 | O43182 | Rho GTPase-activating protein 6 | 105882 | 32 | 4 | 7 | 9% | R.EQQVTQK.K K.DPGMTGSSGDIFESSSLR.A + Phospho (ST) -.MSAQSLLHSVFSCSSPASSSAASAK.G + Deamidated (NQ); Oxidation (M); 3 Phospho (ST) R.EQQVTQKKLSSANSLPAGEQDSPR.L + 2 Phospho (ST) |
|
| ||||||||
| P15 | O76074 | cGMP-specific 3′, 5′-cyclic phosphodiesterase | 99921 | 39 | 5 | 5.74 | 10% | R.WILSVKKNYR.K + Phospho (ST) K.KIAATIISFMQVQK.C + Oxidation (M); Phospho (ST) K.ELNIEPTDLMNREKK.N + Deamidated (NQ) K.TQSILCMPIKNHREEVVGVAQAINK.K + 4 Deamidated (NQ); 2 Phospho (ST) R.GHTESCSCPLQQSPRADNSAPGTPTRK.I + 2 Deamidated (NQ); Phospho (ST) |
|
| ||||||||
| P16 | O60299 | ProSAP-interacting protein 1 | 71747 | 35 | 5 | 7.56 | 9% | K.SRTMTPAGGSGSGLSDSGR.N + Oxidation (M) K.SRTMTPAGGSGSGLSDSGR.N + Oxidation (M); 4 Phospho (ST) R.IGTASYGSGSGGSSGGGSGYQDLGTSDSGR.A + 4 Phospho (ST); Phospho (Y) K.SRTMTPAGGSGSGLSDSGR.N + Oxidation (M); 4 Phospho (ST) K.QLQLSYVEMYQRNQQLER.R + 3 Deamidated (NQ); Phospho (Y) |
|
| ||||||||
| P17 | P16070 | CD44 | 81487 | 79 | 3 | 5.13 | 16% | R.YGFIEGHVVIPR.I R.TPQIPEWLIILASLLALALILAVCIAVNSRRR.C K.SQEMVHLVNKESSETPDQFMTADETRNLQNVDMK.I |