Table 1.
Primers and restriction endonucleases used for SNP genotyping.
| SNP | Primers | Sequence (5′ to 3′) | Restriction endonucleases |
|---|---|---|---|
| TNFRI − 609 G/T, rs4149570 | Forward Reverse |
CGGACGCTTATCTAT ATCTC TTGTAGTCCAGTCACAAGCA |
Bst4C I |
|
| |||
| TNFRI − 1207 C/G, rs4149569 | Forward Reverse |
TTGGGAGATGTCTGCATCAA TTCTTCGTTTGCTTGTTTTTCA |
BstC8 I |
|
| |||
| TNFRII − 1709 A/T, rs652625 | Forward Reverse |
GAGTGCTGAGTGAGAAACTG AGCTTGAATTCGTTCCCAGG |
DseD I |
|
| |||
| TNFRII − 3609 C/T, rs590368 | Forward Reverse |
ATGCTTTTGTCCATGCAGGT GCTGTACCCCGTATTAGCTG |
Msp I |
SNP: single nucleotide polymorphism; TNFR: tumor necrosis factor receptor.