Table 2.
The level of membrane-bound TNFα types I and II receptors expressed on CD3+, CD19+, or CD14+ PBMC subpopulations.
| Percent of cells expressing receptors | Number of receptors per cell | |||
|---|---|---|---|---|
| TNFRI | TNFRII | TNFRI | TNFRII | |
| CD19+ cells | 1.3 (0.9–2.1)* | 7.9 (5.8–12.3)* | 1153.7 (891.9–1490.8)* | 1102.5 (933.4–1309.2)* |
| CD3+ cells | 1.5 (0.9–2.6)* | 36.6 (28.4–47.6)** | 427.3 (349.2–524.7)** | 570.3 (516.8–627.1)** |
| CD14+ cells | 11.4 (4.0–28.8) | 28.3 (12.2–43.3)*** | 869.4 (756.9–1017.9)*** | 1273.9 (1053.0–1450.6)*** |
| Mock-stimulated CD14+ cells1 | 9.8 (5.8–35.0) | 50.0 (31.2–72.1)† | 1267.5 (1053.0–1521.1)† | 1983.4 (1677.9–2268.9)† |
| LPS-stimulated CD14+ cells1 | 9.6 (4.0–32.3) | 79.2 (71.6–87.8) | 1718.2 (1245.0–2559.1) | 4177.0 (3133.1–5173.5) |
Data are expressed as median values (interquartile range).
1Mononuclear cells (2 × 106/mL) were cultured in the absence or presence of LPS (E. coli serotype 055:B5) at a concentration of 200 ng/mL for 24 h. *Significantly different from CD14+ cells (P < 0.001); **significantly different from CD19+ and CD14+ cells (P < 0.001); ***significantly different from CD14+ cells harvested from mock-stimulated PBMCs (P < 0.001); †significantly different from CD14+ cells harvested from LPS-stimulated PBMCs (P < 0.001).