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Persistent acid-α glucosidase (GAA) expression in Gaa−/− XII motoneurons after intralingual injection of AAV9-hGAA or AAV1-hGAA. All tissues were harvested 4 months following AAV delivery to tongue. (a) AAV9-hGAA–treated Gaa−/− mice had more robust GAA expression in the XII motor nucleus as compared to (b) AAV1-hGAA. The average number of GAA-positive cells in the XII nucleus is shown in Panel c (*P < 0.05 compared to AAV9). Scale bars = a,b: 50 µm.
