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. 2014 Mar 11;22(4):862–872. doi: 10.1038/mt.2013.301

Figure 2.

Figure 2

Clinically relevant medium (CRM)–based spheroid culture conditioned medium (CM) increases proliferation and antiapoptotic signaling of human umbilical vein endothelial cells (HUVECs) in vitro. (a) Growth of HUVECs cultured in different CM. The same number of HUVECs was cultured in different CM for 4 days, and the HUVECs grew fastest in the CM (CRM [serum+], spheroid) group (*P < 0.05 compared withto the growth in the CM (CRM [serum+], spheroid) group. (b) Viable cell number as evaluated by Neutral Red assay on day 4. *P < 0.05 compared with the CM (CRM [serum+], spheroid) group. Quantitative data are mean ± SD of at least three independent cultures per group. (c) Metabolic activity evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on day 4. (d) Messenger RNA expression of apoptosis-related factors BCL2 and TP53, analyzed by reverse transcription polymerase chain reaction on day 4.