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. 2014 Feb 25;22(4):828–841. doi: 10.1038/mt.2013.300

Figure 5.

Figure 5

Gint4.T induces GBM cell differentiation. T98G (a) and U87MG (b) cells were either mock-treated or treated with Gint4.T or the unrelated aptamer, used as a negative control, by renewing the aptamer treatment each 24 hours and the cell number was counted at the indicated time points. In (a), at day 4 of Gint4.T treatment, the aptamer was removed from the culture medium (the arrow) and incubation prolonged (dashed line). (a,b) Growth curves represent the average of three independent experiments. ***P < 0.0001; **P < 0.005; *P < 0.05 relative to unrelated (n = 6). Error bars represent mean ± SD. (c) T98G and U87MG cells were treated for 6 days with Gint4.T or the unrelated aptamer and photographed by phase-contrast microscopy (magnification 4×). (d) Cells were treated as in (c) or with 1 µmol/l ATRA and GFAP mRNA levels were analyzed by RT-PCR. ATRA, all-trans retinoic acid; GFAP, glial fibrillary acidic protein.