Figure 2.

Gel electrophoresis images of SMN amplicons. (a) RT-PCR products were amplified using forward primer at exon 6 and reverse primer at exon 8. Bands: upper: amplicons with exon 7; lower: amplicons without exon 7. SCRM: scrambled AON. NC: negative control where cells were transfected with Lipofectamine 2000 only. Lane labels prefixed with “Dual” refer to dual-targeting AONs (see text below). (b) The same gel electrophoresis image with inverted color. The efficiency of each AON in inducing exon 7 inclusion, measured by densitometer comparing upper band and lower band, are presented in % under each corresponding lane. AON, antisense oligonucleotide.