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Fold increase of full-length SMN transcripts measured at various antisense oligonucleotide (AON) transfection concentrations. (a) 25 nmol/l. (b) 50 nmol/l. (c) 100 nmol/l. (d) 200 nmol/l. The fold increase of exon 7 inclusion induced by each AON was normalized with the internal control (GAPDH) and the data was further normalized to the level of the corresponding transcript in the normal control (Lipofectamine2000 only; labeled as NT).
