Figure 2. Effect of HMBA analogs on HEXIM1 expression.
LNCaP cells were treated with vehicle, HMBA (5mM) and its analogs at 500 μM for 24h. Level of HEXIM1 (upper panel) and β-actin (lower panel) was analyzed by Western blot of cell extracts with HEXIM1 antibody and β-actin antibody respectively. The band intensities of HEXIM1 and β-actin were quantified using ImageJ (NIH) and listed below the bands. The results are the representative of three independent experiments.