Figure 3. Lower concentration of HMBA analogs on HEXIM1 expression.
LNCaP cells were treated with vehicle, HMBA (5mM) and identified analogs at 100 μM for 24h. Level of HEXIM1 (upper panel) and β-actin (lower panel) was analyzed by Western blot of cell extracts with HEXIM1 antibody and β-actin antibody respectively. The band intensities of HEXIM1 and β-actin were quantified using ImageJ (NIH) and listed below the bands. The results are the representative of three independent experiments.