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Effect of GCP preparation time on transcript abundance. Long procedure takes >6 h whereas the short procedure can be finished in about 2 h. Transcript abundance of the indicated genes relative to the >6 h procedure was determined by RT-qPCR analysis. Results are shown as mean (n=6) ± standard error. Statistical significance of the difference between means (short versus long procedure) was detected with two-tailed Student’s t-test (*** = P<0.001, ** = P<0.01, * = P<0.05).
