Figure 2.

smc-5 acts in parallel to NER in genome maintenance upon UV irradiation. (A) Slot blot stained with αCPD antibody of whole genomic DNA of young adult worms immediately (0 hr) or 1 day after (24 hr) irradiation with 60 mJ/cm² UVB. From left to right, decreasing amounts of DNA were blotted on the membrane. (B) Germline development quantification of worms 3 days after irradiation with UVB at L1 stage or untreated control worms. Worms were categorized into groups of “normal,” “disrupted,” and “no germline” by inspection on a dissection microscope. (C) Percentage of larval stages 72 hr after UVB irradiation at L1 larval stage of the indicated genotypes. “n” indicates number of animals assessed. A representative experiment is shown.