Table II. Selected metabolite levels of primary metabolism in GT and ROX370.

Data represent means ± sd from five independent experiments. The data of the GT strain were obtained from our previous study (Osanai et al., 2014). Metabolite levels were calibrated relative to that of the GT strain under nitrogen-replete conditions (set at 100%). Statistically significant differences between GT and ROX370 are marked by asterisks (Student’s t test; *P < 0.05, **P < 0.005).

Metabolites	Relative Metabolite Levels
	GT		ROX370
	+Nitrogen	−Nitrogen, 4 h	+Nitrogen	−Nitrogen, 4 h
Sugar metabolism
Glc-1-P	100 ± 29.1	119.1 ± 27.1	87.9 ± 17.8	96.6 ± 20.1
Glc-6-P/Fru-6-P/Man-6-P	100 ± 27.5	148.2 ± 12.6	60.8 ± 10.8*	118.0 ± 16.0*
Glucosamine-6-phosphate	100 ± 39.7	64.7 ± 25.6	99.4 ± 48.7	8.7 ± 5.1**
Gluconate	100 ± 28.9	172.7 ± 10.1	78.5 ± 14.1	118.3 ± 7.4**
GlcNAc	100 ± 35.0	57.1 ± 23.8	159.0 ± 15.3*	54.3 ± 6.6
GlcNAc-6-P	100 ± 45.9	87.2 ± 19.9	87.0 ± 21.6	59.6 ± 21.4*
GDP-Man	100 ± 34.7	168.0 ± 12.7	88.6 ± 26.5	98.1 ± 12.1**
Glycolysis
Fru-1,6-bisP/Glc-1,6-bisP	100 ± 32.4	82.2 ± 6.9	111.3 ± 35.7	150.9 ± 18.1**
Dihydroxyacetone phosphate	100 ± 29.7	149.8 ± 14.3	76.5 ± 9.3	118.6 ± 18.3*
3-Phosphoglycerate	100 ± 28.6	138.6 ± 21.6	112.0 ± 39.2	151.4 ± 31.8
2-Phosphoglycerate	100 ± 53.8	155.0 ± 27.6	107.1 ± 31.9	76.3 ± 13.0**
Phosphoenolpyruvate	100 ± 51.7	104.4 ± 19.5	124.2 ± 41.5	78.4 ± 30.8
Acetyl-CoA	100 ± 38.3	83.1 ± 23.6	90.8 ± 30.9	108.5 ± 35.7
OPP pathway
Sedoheptulose-7-phosphate	100 ± 35.9	121.0 ± 5.8	70.3 ± 12.9	107.1 ± 9.6**
Tricarboxylic acid cycle
Citrate	100 ± 32.4	59.1 ± 38.8	91.0 ± 25.9	92.4 ± 29.1
Cis- and trans-aconitate	100 ± 53.7	114.8 ± 55.4	53.9 ± 28.7	46.4 ± 25.8
Isocitrate	100 ± 82.7	197.0 ± 157.7	31.2 ± 29.0	28.0 ± 29.0
Succinate	100 ± 23.9	398.3 ± 94.0	97.0 ± 19.5	355.6 ± 39.4
Fumarate	100 ± 57.0	1,014.8 ± 93.1	146.0 ± 89.3	386.7 ± 58.0**
Malate	100 ± 40.9	875.6 ± 83.4	172.3 ± 83.1	372.9 ± 52.6**