Skip to main content
. 2014 Feb 13;164(4):1879–1892. doi: 10.1104/pp.113.233031

Figure 2.

Figure 2.

Interspecies and intraspecies interactions between DCP1 and myosins (related to Table I). A, Yeast two-hybrid interactions. Top, Double-transformed yeast cells on selective dropout (SD) medium lacking Leu (−L) and Trp (−W). Bottom, Interaction between the Arabidopsis AtDCP1, yeast DCP1p, and human DCP1a and DCP1b N-terminally fused to the GAL4-AD and the tails of yeast Myo2p and mouse MyoVa, N-terminally fused to the GAL4-BD, on selective dropout medium lacking Leu, Trp, and His (−H), supplemented with 3 mm 3-aminotriazole (3AT). GFP N-terminally fused to the GAL4-AD was included as a negative control. B, Coprecipitations. a, Input of GST-DCP1p-His6 (left gel; *, approximately 56 kD; 0.18 µg), no coprecipitation of GST-DCP1p-His6 with MBP (7.5 µg) as a negative control (other gels) and coprecipitation with MBP-Myo2p (6.9 µg) detected by anti-His6 antibody staining. b, Input of MyoVa-His6 (left gel; *, approximately 84 kD; 2.9 µg), no coprecipitation of MyoVa-His6 with MBP (7.5 µg) as a negative control (other gels) and coprecipitation with MBP-DCP1a (4.2 µg) detected by anti-His6 antibody staining. c, Input of MyoVa-His6 (left gel; *, approximately 84 kD; 2.9 µg), no coprecipitation of MyoVa-His6 with MBP (7.5 µg) as a negative control (other gels) and coprecipitation with MBP-DCP1b (4.4 µg) detected by anti-His6 antibody staining. d, Input of GST-AtDCP1-His6 (left gel; *, approximately 68 kD; 2.3 µg), no coprecipitation of GST-AtDCP1-His6 with MBP (7.5 µg) as a negative control (other gels) and coprecipitation with MBP-MyoVa (4.4 µg) detected by anti-His6 antibody staining. e, Input of MyoVa-His6 (left gel; *, approximately 84 kD; 2.9 µg), no coprecipitation of MyoVa-His6 with MBP (7.5 µg) as a negative control (other gels) and coprecipitation with MBP-DCP1p (4.3 µg) detected by anti-His6 antibody staining. f, Input of GST-AtDCP1-His6 (left gel; *, approximately 68 kD; 2.3 µg), no coprecipitation of GST-AtDCP1-His6 with MBP (7.5 µg) as a negative control (other gels) and coprecipitation with MBP-Myo2p (6.9 µg) detected by anti-His6 antibody staining. g, Input of GST-DCP1a-His6 (left gel; *, approximately 93 kD; 2.9 µL), no coprecipitation of GST-DCP1a-His6 with MBP (7.5 µg) as a negative control (other gels) and coprecipitation with MBP-Myo2p (6.9 µg) detected by anti-His6 antibody staining. W, Last wash fraction; B, boiled beads.