Figure 3.

BiFC interaction of AtDCP1 and myosin class XI tails at P-bodies. Interaction is shown for myosin tails and AtDCP1 (column I) at AtDCP2-labeled P-bodies in transiently transformed N. benthamiana leaves 48 h posttransfection. Column II shows AtDCP2-mCherry-labeled P-bodies, column III shows the overlay between the images in column I (green) and column II (magenta), and column IV shows the corresponding transmission images. The interaction between RabD1 and the tails of XI-1 was included as a positive control (Supplemental Fig. S2). Plasmids encoding the N-terminal half of YFP alone were included as negative controls for the myosin tails fused to the C-terminal residues of YFP. RabD1 fused to the C-terminal residues of YFP was used as a negative control for AtDCP1 fused to the N-terminal residues of YFP (Supplemental Fig. S3; Tables II and III). Bars = 50 µm.