Yeast strains Y187::ProOskn2-662 and Y187::ProOskn2-D and control strain YPO101 harboring pACTII, pACTII-AtGRF4, pACTII-AtGRF5, pACTII-AtGRF6, and pACTII-OsGRF3 were examined for growth on medium containing His or medium supplemented with 10 mm
3-AT (without His). All GRF constructs show growth on 3-AT containing medium when grown in strains with constructs ProOskn2-662, ProOskn2-B, or ProOskn2-D but not in the control strain YPO101 that contains a promoterless HIS3 reporter. ProOskn2-C is only activated by pACTII-AtGRF5. Results are based on Supplemental Figure S3.