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. 2014 Feb 14;164(4):1952–1966. doi: 10.1104/pp.113.222836

Table III. Interaction of OsGRF3 and Arabidopsis GRF proteins with the Oskn2 promoter and subfragment D.

Yeast strains Y187::ProOskn2-662 and Y187::ProOskn2-D and control strain YPO101 harboring pACTII, pACTII-AtGRF4, pACTII-AtGRF5, pACTII-AtGRF6, and pACTII-OsGRF3 were examined for growth on medium containing His or medium supplemented with 10 mm 3-AT (without His). All GRF constructs show growth on 3-AT containing medium when grown in strains with constructs ProOskn2-662, ProOskn2-B, or ProOskn2-D but not in the control strain YPO101 that contains a promoterless HIS3 reporter. ProOskn2-C is only activated by pACTII-AtGRF5. Results are based on Supplemental Figure S3.

Yeast Strain pACTII
pACTII-AtGRF4
pACTII-AtGRF5
pACTII-AtGRF6
pACTII-OsGRF3
His 3-AT His 3-AT His 3-AT His 3-AT His 3-AT
ProOskn2-A + + + + +
ProOskn2-B + + + + + + + + +
ProOskn2-C + + + + + +
ProOskn2-D + + + + + + + + +
ProOskn2-662 + + + + + + + + +
YPO101 + + + + +