Table IV. Analysis of DNA-binding properties of the QLQ and WRC domains from OsGRF10 in yeast one-hybrid screens.
Yeast strains Y187::ProOskn2-662 and Y187::ProOskn2-D harboring pACTII-QLQ, pACTII-AtGRF4WRC, or pACTII-QLQ-WRC were examined for growth on medium containing His or medium supplemented with 10 mm 3-AT (without His). Individual QLQ and WRC domains cannot activate the ProOskn2:HIS3 reporters when fused to the Gal4p AD in pACTIIa, whereas the combination QLQ-WRC can. Results are based on Supplemental Figure S2C.