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. 2014 Feb 24;164(4):1967–1990. doi: 10.1104/pp.113.225920

Figure 5.

Figure 5.

Overexpression of SlZF2 enhances salinity sensitivity in Arabidopsis. A, Transfer DNA (T-DNA) insertion in the Arabidopsis genome and expression of SlZF2 in L5, L6, and L9 transgenic lines and a control line transformed with empty vector (V) were analyzed by PCR. B, Phenotype of transgenic Arabidopsis plants grown on one-half-strength MS medium and then transferred to soil, and ectopically expressing SlZF2 (Ba, Bc, Be, Bg, and Bh) compared with control plants (Bd and Bf). C, Phenotype of Arabidopsis V (Ca), L5 (Cb), L6 (Cc), and L9 (Cd) lines 5 d after transfer on 125 mM NaCl medium. D, Pro accumulation in control (V) and SlZF2 transgenic lines grown for 5 d on 80 mm NaCl. E, MDA accumulation in control (V) and SlZF2 transgenic lines grown 5 d on 80 mm NaCl. Data represent means and se of three replicates. Letters indicate values that differ significantly between SlZF2-transgenic Arabidopsis and control V lines according to the Student-Newman-Keuls test at P < 0.05.