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. 2014 Feb 25;164(4):2011–2019. doi: 10.1104/pp.113.234401

Figure 6.

Figure 6.

GA-insensitive mutants are hypersensitive to LT. A, Representative features (pistil, anthers, and pollen) are shown in spikelets of the wild-type (WT) TC65, in spikelets of the GA-insensitive gid1-8 mutant, and in spikelets of the SLR1 gain-of-function slr1-d1 and slr1-d3 mutants in the TC65 genetic background. Spikelets were dissected at the heading period with (LT; blue frames) or without (cont.; orange frames) LT treatment. Bars = 1 mm. B, The number of mature pollen grains was severely reduced in the gid1 and slr1-d mutants compared with their parental wild-type TC65 plants. Bars = 50 μm. C, Quantification of the number of mature pollen grains in the mutants and the TC65 control with (blue columns) and without (orange columns) LT treatment (n = 36 anthers tested in each). D and E, Quantification of the number of mature pollen grains in the slr1-d2 mutant and its parental cv Kinmaze (D), and in the slr1-d4 mutant and its parental cv Nipponbare (E), with (blue columns) and without (orange columns) LT treatment (n = 36 anthers tested in each). Values are means ± se. Letters denote statistical differences with Tukey’s test (P < 0.05).