Figure 1.

WRKY45 responds to Pi starvation and is localized in the nucleus. A, qRT PCR analysis of WRKY45 expression. Seven-day-old wild-type seedlings were transferred to LP medium, and then the shoots and roots were harvested separately at the indicated time. The data represent the mean values of three replicates ± se. B, GUS staining assay of the ProWRKY45:GUS transgenic line. Seven-day-old ProWRKY45:GUS seedlings were transferred to MS (a and b) or LP (c and d) medium for 5 d and then stained. Details of the roots of the ProWRKY45:GUS transgenic seedlings are shown in b and d. C, Subcellular localization of the WRKY45:GFP fusion protein in the N. benthamiana leaf. The expression of GFP alone was used as the control. D, Deduced amino acid sequence of WRKY45 showing the highly conserved WRKY domain WRKYGQR and the novel C2H2 zinc finger motif in magenta and blue letters, respectively.