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. 2014 Feb 28;164(4):2020–2029. doi: 10.1104/pp.113.235077

Figure 2.

Figure 2.

Overexpression of WRKY45 enhances Arabidopsis Pi accumulation and Pi uptake. A, qRT PCR analysis of WRKY45 expression in the WRKY45 RNAi lines, WRKY45-overexpressing lines, and wild-type plants. Seven-day-old seedlings were transferred to MS or LP medium and then harvested for RNA extraction. The data are the mean values of three replicates ± se. B, Pi content analysis. Seven-day-old seedlings were transferred to MS or LP medium for 10 d and then harvested for Pi content analysis. Data are shown as mean ± se (n = 3). Asterisks indicate statistically significant differences compared with the wild type (Student’s t test, P < 0.05). Wild-type plants were used as a control (#). C, Pi uptake was monitored over a 2-h period in 7-d-old seedlings germinated and grown on MS medium. Data are shown as mean ± se (n = 3). Asterisks indicate statistically significant differences compared with the wild type (Student’s t test, P < 0.05). Wild-type plants were used as a control (#). WT, Wild type.