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. 2014 Feb 28;164(4):2020–2029. doi: 10.1104/pp.113.235077

Figure 3.

Figure 3.

WRKY45 promotes PHT1;1 expression. A, qRT PCR analysis of PHT1;1 and PHT1;4 expressions in the roots of the WRKY45-overexpressing lines, WRKY45 RNAi lines, and wild-type plants. The plants were germinated and grown on MS medium for 7 d, and then the roots were harvested for RNA extraction. The data are the mean values of three replicates ± se. B, qRT PCR analysis of PHT1;1 expression in the roots of the WRKY45 RNAi lines and wild-type plants. The 7-d-old seedlings were transferred to LP medium for 3 d and then harvested for RNA extraction. The data are the mean values of three replicates ± se. C, GUS staining of the ProPHT1;1:GUS transgenic line. Seven-day-old ProPHT1;1:GUS seedlings were transferred to MS (a and b) or LP (c and d) medium for 5 d and then stained. Details of the roots of the ProPHT1;1:GUS transgenic line are shown in b and d. D, GUS staining showing the expression patterns of PHT1;1 in the WRKY45 RNAi line, WRKY45-overexpression lines, and wild-type plants. The plants were germinated and grown on MS medium for 7 d and then harvested for GUS staining. WT, Wild type.