Figure 6.

The Pi uptake enhancement caused by WRKY45 overexpression is suppressed in the pht1;1 mutant. A, qRT PCR analysis of PHT1;1 or WRKY45 expression in the 35S:WRKY45-18::pht1;1 lines and wild-type seedlings. Seven-day-old seedlings were used for RNA extraction. The data are the mean values of three replicates ± se. B, Pi uptake was monitored over a 2-h period in 7-d-old seedlings germinated and grown on MS medium. Data are shown as mean ± se (n = 3). Asterisks indicate statistically significant differences compared with the wild type (Student’s t test, P < 0.05). Wild-type plants were used as a control (#). WT, Wild type.