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Thr-211 and Thr-212 in the C terminus of SCaBP1 are important for activation of PKS24. A, Activation of PKS24 by SCaBP1, SCaBP1S216A, and SCaBPDN30 but not by SCaBP1N210. Coomassie Blue staining (top) and kinase activity (bottom). B, Activation of PKS24 by SCaBP1, SCaBP1T212A, or SCaBP1T213A. Coomassie Blue staining (top) and kinase activity (bottom). PKS24, SCaBP1, and its variations were fused to GST tag. SC1, SCaBP1.
