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Agarose gel showing ethidium bromide-stained electrophoresis of PCR products of the SRY gene fragment, which was amplified from DNA isolated from paraffin-embedded tissue. Lane M is a ladder. Lane P is a positive control from the spleen of a male pig. Lane C is a sample from the muscular tissue of the present pig. Lane F is a sample from the spleen of a female pig. The arrow indicates 160 bp. A positive band was observed in lane P and lane C.
