Table 1. Oligonucleotide sequences of primers used in this study.
| Primer name | Oligonucleotide (5’→3’) | Reference |
|---|---|---|
| PCR amplification for a partial gltA sequence | ||
| CS7F2 | ATGR*TAGAAAAW*GCTGTTTT | [21] |
| HG1085R | ACTATACCK*GAGTAAAAGTC | [10] |
| F1b | GATCATGAR*CAR*AATGCTTC | [9] |
| AnaCS1085R1 | ACTATACCK*GAGTAAAAR*TC | This study |
| PCR amplification for a partial groELsequence | ||
| EEgro1F | GAGTTCGACGGTAAGAAGTTCA | [3] |
| Anagro712R | CCGCGATCAAACTGCATACC | [21] |
| Anagro122F | AAATACGGTW*GTCACGGG | This study |
| Anagro649R | CTTTCTTCR*ACAGTTATAAG | This study |
| Genome walker gene-specific primers (gltA) | ||
| ABgl-46R1 | AATGCAGCTGCTCCCGCACTTAAGCAAGTA | This study |
| ABgl-1R2 | GTTGAGCCCACCATTCTTACTGTAGATGTA | This study |
| ABgl-307F1 | CAATATAGCGATCGCTATGGAAGAAATAGC | This study |
| ABgl-338 F2 | TTGCAGGATGAATACTTCATAGAGAGGAAG | This study |
| Genome walker gene-specific primers (groEL) | ||
| ABgr-361F1 | TAAAGGCAAAAGAGGCTGTTCTTACGG | This study |
| ABgr-385F2 | CGGC TCTT ATGT CCAT GAGA CGTG AA | This study |
| ABgr-1010GRF3 | ACAGTGCATCTTCCAGCATAGAAAGTAG | This study |
| ABgr-1117F4 | AGCTTTCAGGTGGTGTGGCTGTGCTGAAAG | This study |
| ABgr-1132F5 | TGGCTGTGCTGAAAGTTGGTGGATCAAGTGA | This study |
| ABgr-96R1 | TTAGGTCCAGCAGTACATCCAACAGCA | This study |
| ABgr-30R2 | GGATGTGTACTATCTCCCTAATGGCTTA | This study |
| ABgr-25R3 | TCTCCCTAATGGCTTTATCCAACTTCTC | This study |
*Degenerate primers: R=A or G, W=A or T, K=G or T