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. 2013 Nov 1;76(2):301–306. doi: 10.1292/jvms.13-0378

Table 2. Primers Used for qRT-PCR.

Gene Primer setID F/R Sequence Tm (°C) Amplicon Size (bp)
GUSB DA021273 F 5’-ACATCGACGACATCACCGTCA-3’ 76 90
R 5’-GGAAGTGTTCACTGCCCTGGA-3’
TBP DA049514 F 5’-ATGGTGTGTACGGGAGCCAAG-3’ 76 184
R 5’-ACTGTTGGTGGGTCAGCACAAG-3’
VEGF-A DA027420 F 5’-TCAGGACACTGCTGTACTTTGAGG-3’ 79.1 133
R 5’-GGCTTGTCAGGAGCAAGTGAA-3’
VEGFR-1 DA035194 F 5’-CACCTGGGCTGAGAGCAAAC-3’ 77.2 117
R 5’-CCACACCTGGAATGGCAGAA-3’
VEGFR-2 DA022900 F 5’-CTTGGACAGCATCACCAGTAGTCAG-3’ 75 131
R 5’-TGAGATGCTCCAAGGTCAGGAA-3’
VEGFR-3 DA049880 F 5’-GGATGGAGTTCCTGGCCTCA-3’ 77.9 142
R 5’-TTTCGCACATAGTCAGGGTCTTTG-3’
PDGF-B DA006888 F 5’-TACGAGATGCTGAGCGACCAC-3’ 79.1 112
R 5’-ATCGGGTCAAATTCAGGTCCAA-3’
PDGFR-β DA076806 F 5’-TGAGGGCAAGCTGGTCAAGA-3’ 77.7 155
R 5’-ACACGTCGCTCAGGGTGGTA-3’
EGF DA074730 F 5’-CTATGGCCCTCAAGGATGGTG-3’ 75.1 125
R 5’-GCAGCCTTGCTCTGTGTCCTTA-3’
TGF-α DA057929 F 5’-GTGGTGTCCCACTTCAACGACT-3’ 77 85
R 5’-TGTCCTCCTGCACCAGAAACC-3’
HB-EGF DA044315 F 5’-GCTCTGGCCACACCCAGTAA-3’ 75.3 113
R 5’-CCATGGATGCAGAAGTCCTTGTA-3’
EGFR DA000951 F 5’-TGCATTTGCCAAGCCCTACA-3’ 75.1 90
R 5’-GGTACTCGTCAGCATCCACAACA-3’
HGF DA071494 F 5’-GGCTACTGCTCCCAAATTCCA-3’ 71.9 124
R 5’-CCCACATTGAACATGTTAGTCCAGA-3’
MET DA075812 F 5’-ACCAGTGAAGTGGATGGCTTTAGAA-3’ 75.5 135
R 5’-AAGGTGTTGACGTCAGGATAAGGTG-3’
IGF1 DA020266 F 5’-GATAGAGCCTGCGCAATGGAA-3’ 71.3 120
R 5’-CTGGAAATGAATTGGTTAGCAGGAA-3’
IGF1R DA026006 F 5’-ATGGTGGCCGAAGATTTCACA-3’ 75.1 120
R 5’-AGGTGACATCCAGCGCACAG-3’
IGF2R DA044849 F 5’-CACAGTGCGTGACTTACGGAAAC-3’ 76.2 84
R 5’-CCAGAGCAGGAATCCCAAGTG-3’
Ang-2 DA059135 F 5’-TGGGTGGACGGTCATTCAG-3’ 74.7 106
R 5’-CAGCCAGTGTTCGCCAGAAG-3’
Tie2 DA084660 F 5’-GCGGGAATGACTTGCCTGA-3’ 76.9 146
R 5’-AGGGCCAGAGTTCCTGAGTTGA-3’