Fig. 4.

Effect of COX-2 selective inhibitor NS-398 on the expression of c-Fos protein in the paraventricular nucleus (PVN) after LPS injection. Vehicle (A–C) or COX-2 selective inhibitor NS-398 (10 mg/kg BW, ip, D–F) was injected to male rats 30 min before applying LPS (100 µg/kg BW, ip). Scale bars: 100 µm. Quantification of the number of c-Fos-IR cells in the PVN of vehicle-treated (closed column) or NS-398-treated (open column) rats after an injection of LPS (G). Each column with a vertical bar represents the mean ± SEM (n=5). Values with different letters are significantly different (P<0.05, two-way ANOVA followed by Tukey’s test).