Fig. 8.

Effect of COX-2 selective inhibitor NS-398 on the expression of c-Fos protein in the supraoptic nucleus (SON) after 2DG injection. Vehicle (A–C) or COX-2 selective inhibitor NS-398 (10 mg/kg BW, ip, D–F) was injected to male intact rats 30 min before applying 2DG (400 mg/kg BW, ip). Scale bars: 100 µm. Quantification of the number of c-Fos-IR cells in the SON of vehicle-treated (closed column) or NS-398-treated (open column) rats after an injection of 2DG (G). Each column with a vertical bar represents the mean ± SEM (n=5). Values with different letters are significantly different (P<0.05, two-way ANOVA followed by Tukey’s test).