Fig. 2.

PRC regimen does not modify blood glucose levels but modulate circulating IGF-1 and IGFBPs. (A) Blood glucose levels are expressed as concentration (mg dL⁻¹). No significant difference was detected between the experimental groups (6–13 samples per group). (B–D) Mouse Serum IGF-1 and IGFBP-1/3 levels are expressed as concentration (ng mL⁻¹) (3–7 samples per group). (B) WT mice sacrificed at the end of PR diet cycle displayed significantly lower IGF-1 levels when compared with corresponding control group (*P < 0.05). 3xTg-AD mice showed a significant reduction in IGF-1 levels not only during the PR diet cycle but also during the normal diet refeeding (***P < 0.001). We detect a significant difference between WT control and 3xTg-AD control groups (##P < 0.01). (C) 3xTg-AD mice showed a significant reduction in IGFBP-3 levels not only when fed with the PR diet (***P < 0.001) but also during the refeeding cycle (**P < 0.01). (D) We determined a significant increase in IGFBP-1 levels at the end of the PR cycle in 3xTg-AD mice (**P < 0.01).