PRC regimen alleviates age-dependent behavioral changes in 3xTg-AD mice. (A and B) Shown is SAB (spontaneous alternation behavior) percentage, obtained testing the mice with the Y-maze test at 8–9 months of age, before any dietary treatment (A), or at 12.5–13.5 months of age, after 18 weeks of PRC regimen (B). (A) 3xTg-AD mice already showed working memory impairment performing significantly worse than WT control group (*P < 0.05, 13–14 mice per group). (B) Only 3xTg-AD control group performed worse than WT control (*P < 0.05, 13–14 mice per group). (C) NOR test was used to calculate RI (recognition index). RI scored for 3xTg-AD control animals was significantly lower than values calculated for WT control (*P < 0.05, 12–14 mice per group). (D) EPM test was used to score the time spent by the rodents in open arms. No significant difference was detected between the experimental groups (13–14 mice per group).