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. Author manuscript; available in PMC: 2014 Sep 20.
Published in final edited form as: Oncogene. 2013 Apr 1;33(12):1590–1600. doi: 10.1038/onc.2013.92

Figure 4.

Figure 4

PIK3CA mutation but not PTEN loss sensitizes KRAS mutant cells to PP242 (A) siRNA against PTEN does not sensitize KRAS mutant CRC cells to PP242. SW620 cells were treated with siRNA against PTEN for 72 hours prior to 1 hour drug treatment with PP242 (3.0, 1.0, 0.33 and 0.1 μM). (B) Addition of mutant PIK3CA to the KRAS mutant cell line SW620 increases sensitivity to PP242. Retroviral insertion of either WT PIK3CA, helical (E542K) or kinase (H1047R) domain mutations only resulted in elevated basal AKT activation in the H1047R case. Cells were treated with rapamycin (20 nM) or PP242 (3.0, 1.0, 0.3 μM) for 1 h before lysis. (C) IC50s for SW620 cell lines engineered to contain additional PIK3CA mutations.