Figure 2. Characterization and transgenic targeting of LS Crfr2α⁺ neurons.

(A–E) Crfr2 mRNA ISH; coronal diagrams in (A) depict region analyzed.

(F) dFISH for Crfr2 (green) and Gad2 (red).

(G,H) dFISH for Crfr2 (green) vs Esr1 (red): Crfr2⁺/DAPI⁺ = 8.3%, Esr1⁺/DAPI⁺ = 1.7%, Crfr2⁺/Esr1⁺ = 0, Esr1⁺/Crfr2⁺ = 0; total n = 11,086 DAPI⁺ cells

(I,J) dFISH for Crfr2 (green) vs Penk (red): Crfr2⁺/DAPI⁺ = 9.3%, Penk⁺/DAPI⁺ = 3.3%, Crfr2⁺/Penk⁺ = 0.1%, Penk⁺/Crfr2⁺ = 0.4%; total n = 8,274 DAPI⁺ cells

(K) Crfr2α-eGFPCre BAC transgene design.

(L,M) dFISH, Cre mRNA (green) vs. Crfr2 mRNA (red): Crfr2⁺/DAPI⁺ = 9.3%, Cre⁺/DAPI⁺ = 3.7%, Cre⁺/Crfr2⁺ = 34.4%, Crfr2⁺/Cre⁺ = 86.6%; total n = 17,537 DAPI⁺ cells

(N,O) eGFPCre⁺ nuclei (green) vs. UCN3 pericellular baskets (pcbs; red); counts from areas with detectable transgene expression. UCN3⁺ pcbs colocalize with synaptophysin (data not shown). eGFPCre⁺/DAPI⁺ = 12.8%, UCN3pcb⁺/DAPI⁺ = 11.8%, UCN3pcb⁺/eGFPCre⁺ = 65.2%, eGFPCre⁺/UCN3pcb⁺ = 71.0%; total n = 2,665 Nissl⁺ cells

(P) Injection site (left) and Cre-dependent AAV (right).

(Q,R) Transgenic eGFPCre⁺ nuclei (green) vs. recombined, viral tdT (red); counts from areas containing tdT⁺ cells. eGFPCre⁺/DAPI⁺ = 11.7%, tdT⁺/DAPI⁺ = 6.3%, tdT⁺/eGFPCre⁺ = 42.1%, eGFPCre⁺/tdT⁺ = 77.9%; total n = 3,586 DAPI⁺ cells

(S,T) dFISH for Crfr2 mRNA (green) vs. tdT mRNA (red); counts from areas containing tdT⁺ cells. Crfr2⁺/DAPI⁺ = 12.5%, tdT⁺/DAPI⁺ = 7.1%, tdT⁺/Crfr2⁺ = 51.9%, Crfr2⁺/tdT⁺ = 91.6%; total n = 5,829 DAPI⁺ cells

In this and subsequent figures, dashed lines at left indicate lateral ventricle (LV), boxed regions shown at higher magnification in insets.

See also Figures S1 and S2.