Figure 1. Newborn rat cardiomyocyte culture treated with oxytocin release atrial natriuretic peptide (ANP) to the medium and accumulate ANP in the perinuclear region of the cells. A, Radioimmunoassay of ANP from cultures treated with different oxytocin concentrations for 24 h. *P<0.05, compared to control (one-way ANOVA). B, Control cardiomyocyte culture stained with specific marker, troponin T (red) and nuclear Dapi stain (blue). C, Cardiomyocytes treated for 24 h with 10 pM oxytocin expressed green fluorescence specific for ANP.
