Figure 3. Effects of chronic unpredictable mild stress (CUMS), fluoxetine (FLX), and re-exposure to CUMS on the levels of tau and phospho-tau in the hippocampi and the frontal cortex of rats. GAPDH was used to normalize the quantification in Western blot experiments. Representative Western blot bands of the hippocampi (A) and the frontal cortex (B) and densitometric analyses of the bands for p-Tau (Ser356) and p-Tau (Thr231) ratio of the hippocampi, and p-Tau (Ser356) and p-Tau (Thr231) ratio of the frontal cortex. Cont: Controlvehicle (V) CUMS: CUMSV FLX: CUMSFLX Re-CUMS: CUMSFLXCUMS. Data are reported as meansSD. P0.01 (one-way ANOVA followed by the Bonferroni test).