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The levels of Arf6-GTP in platelets from different species. (A) Washed platelets (2 ×108) were incubated at 37°C for 5 min, lysed, and analysed for Arf6-GTP as in Methods. An aliquot of each lysate (5%) was immunoblotted for Arf6 or RabGDIα(Total). (B) The ratios of Arf6-GTP to Arf6 total were calculated from fluorescent intensities. (C) The ratios of Arf6-GTP to RabGDIα totals were calculated and normalized to human #1.
