Table II.
Protein expression in platelets from different species.
| Proteins Species |
OCS+
|
OCS−
|
p-value# | ||||
|---|---|---|---|---|---|---|---|
| Human | Murine | Canine | Bovine | Camelid | Equine | ||
| Arf6 | 1.74 (±0.45) | 1.19 | 0.84 | 0.37 | 1.03 | 0.52 | 0.1336 |
| β-Actinin | 0.43 (±0.02) | 0.27 | 0.16 | 0.06 | 0.06 | 0.12 | 0.0627 |
| FAK | 0.64 (±0.07) | 0.37 | 0.54 | 0.43 | 0.31 | 0.29 | 0.1258 |
| Arf1/3 | 0.81 (±0.25) | 0.50 | 0.47 | 0.39 | 0.38 | 0.46 | 0.1734 |
| Cdc42 | 0.58 (±0.30) | 0.30 | 0.23 | 0.14 | 0.16 | 0.08 | 0.0881 |
| Rac1 | 1.79 (±0.28) | 2.44 | 2.29 | 1.07 | 1.14 | 1.01 | 0.0055 |
| RhoA | 0.56 (±0.28) | 0.38 | 0.62 | 0.25 | 0.31 | 0.31 | 0.0368 |
| Rap1 | 2.28 (±0.30) | 1.67 | 1.83 | 1.41 | 1.50 | 1.39 | 0.0586 |
| RalA | 1.12 (±0.25) | 0.72 | 1.62 | 0.67 | 0.95 | 0.96 | 0.3487 |
| Ras-K, H, or N | 0.31 (±0.15) | 0.27 | 0.26 | 0.18 | 0.20 | 0.21 | 0.0093 |
| CaM | 0.40 (±0.11) | 0.26 | 0.40 | 0.20 | 0.21 | 0.20 | 0.0332 |
| Src(pp60) | 0.93 (±0.05) | 0.69 | 0.76 | 0.21 | 0.51 | 0.49 | 0.0315 |
| β3 integrin | 0.77 (±0.03) | 0.59 | 0.37 | 0.59 | 0.49 | 0.53 | 0.7691 |
| IQGAP1 | 0.09 (±0.03) | 0.35 | 0.71 | 0.84 | 0.85 | 0.60 | 0.1276 |
| IQGAP2 | 0.62 (±0.08) | 0.04 | 0.49 | 0.03 | 0.28 | 0.29 | 0.3932 |
| GIT1 | 0.08 (±0.03) | 0.03 | 0.02 | 0.00 | 0.00 | 0.01 | 0.1328 |
| ASAP1 | 0.19 (±0.09) | 0.07 | 0.18 | 0.02 | 0.03 | 0.07 | 0.0554 |
The ratios of different proteins to RabGDIα were calculated from the fluorescent intensities of the immuno-decorated proteins detected in Figure 2. The human values were averaged from the five samples. The standard deviations from the five human samples (Figure 2) were calculated and shown in parenthesis.
#
To evaluate correlations, the ratios were grouped as OCS+ and OCS− species, averaged, and the significance of the differences between averages were tested by unpaired Student’s t-test. p values<0.05 were considered to be significant (bold).