Figure 2.

Evaluation of the stability of the 15 candidates for signature peptides (SP) from the anti-HCV mAb, by continuous incubation of the digest mixture under conditions mimicking (A) prolonged tryptic digestion (37 °C, pH 8.5, 24 h) and (B) queuing in a cooled autosampler (4 °C, pH 2.8, 48 h), respectively. The peptides are represented by the first three residues of their sequence (the full list is in Table 1 of the Supporting Information). GPS and TVA peptides from the heavy and light chains of the mAb, respectively, were finally selected as the SP (shown in red and blue).