Table 1. Pharmacokinetic Parameters Calculated Based on the Quantitative Results Obtained by Different Calibration Approaches and Signature Peptides.
| AUC (μg h/mL) | Cl (mL/h) | Vss (mL) | T1/2 (h) | |
|---|---|---|---|---|
| protein calibration, GPS | 7274 | 0.083 | 28.9 | 260.7 |
| protein calibration, TVA | 7113 | 0.085 | 28.8 | 260.8 |
| aprotein calibration, mean of two SP | 7194 | 0.084 | 28.9 | 260.9 |
| protein calibration, deviation between the two SP | 2% | 2% | 0% | 0% |
| hybrid no. 2 (SIL-extended-peptide I.S.), GPS | 7551 | 0.080 | 30.6 | 298.5 |
| hybrid no. 2 (SIL-extended-peptide I.S.), TVA | 6763 | 0.089 | 32.3 | 263.9 |
| hybrid no. 2, mean of two SP | 7155 | 0.084 | 31.4 | 281.2 |
| hybrid no. 2, deviation between the two SP | 8% | 8% | 4% | 9% |
| hybrid no. 1 (SIL-peptide I.S.), GPS | 7244 | 0.083 | 27.9 | 252.5 |
| hybrid no. 1 (SIL-peptide I.S.), TVA | 7125 | 0.085 | 28.9 | 261.1 |
| hybrid no. 1, mean of two SP | 7212 | 0.084 | 28.3 | 252.5 |
| hybrid no. 1, deviation between the two SP | 1% | 1% | 2% | 2% |
| extended-peptide calibration, GPS | 5774 | 0.105 | 38.2 | 271.1 |
| extended-peptide calibration, TVA | 3696 | 0.163 | 49.5 | 239.2 |
| extended-peptide calibration, mean of two SP | 4721 | 0.128 | 43.2 | 255.6 |
| extended-peptide calibration, deviation between SP | 31% | 31% | 18% | 9% |
| peptide calibration, GPS | 5640 | 0.107 | 35.9 | 252.5 |
| peptide calibration, TVA | 2995 | 0.202 | 74.7 | 280.7 |
| peptide calibration, mean of two SP | 4293 | 0.141 | 49.0 | 260.4 |
| peptide calibration, deviation between the two SP | 43% | 43% | 50% | 7% |
a
Parameters were calculated based on the mean of the concentrations obtained by the two SP.