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. 2014 Apr 15;25(8):1298–1311. doi: 10.1091/mbc.E13-11-0687

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© 2014 Connolly et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Assembly of monopolar oocyte meiotic spindles in klp-18 mutant requires both the katanin activity of MEI-1 and ASPM-1. (A) Representative spinning-disk confocal images were recorded over time during meiosis I in live mutant embryos (Supplemental Movies S6–S9 and S13–S18) expressing mCherry:Histone2B and GFP:β-tubulin translational fusions to mark chromosomes and microtubules, respectively, with the exception of klp-18(or447ts) oocytes, which expressed GFP:Histone2B only. The images shown are from ovulation and from the time during meiosis I at which the chromosomes occupied the smallest area. (B) Scatter plot showing the ratios of the areas occupied by chromosomes at ovulation over the smallest areas occupied during meiosis I. For mei-1(-) and klp-18(-), measurements were taken using RNAi (black dots) and ts mutations (red dots) to reduce gene function. Average areas for combined genotypes at ovulation (O), average smallest areas (S), average ratios (O/S), and number of embryos analyzed for each genotype (N) are shown below the graph. (C) Bar graph showing the average ratio of the areas occupied at ovulation over the smallest area occupied during meiosis I for selected genotypes. The p values from Student's test are indicated in black for observations made in the mei-1(ct46ct103) background, in red for tba-2(sb27);tbb-2(sb26) background, and in blue when comparing these two when klp-18 is knocked down. p < 0.05 indicates a statistically significant difference.