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. 2014 Apr 15;25(8):1327–1337. doi: 10.1091/mbc.E13-08-0447

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© 2014 Jiang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Knockdown of VPS33A and UVRAG causes accumulation of autophagosomes and endolysosome-like structures. (A) HeLa cells treated with siRNA against luciferase, VPS33A, STX17, or UVRAG were cultured in starvation medium for 1 h and subjected to conventional electron microscopy analysis. White arrows indicate autophagosomes; black arrows indicate electron-dense endolysosome-like structures. Scale bars, 500 nm. (B) Quantification of the number of autophagosomes and high-density organelles from at least 50 randomly selected areas.