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. 2014 Apr 15;25(8):1366–1373. doi: 10.1091/mbc.E13-09-0527

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© 2014 Liu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — NoTS assay revealed that the initiation of photobodies follows a self-organization model. (A) COP1, PIF7, phyB, cry1, and cry2 were able to form nuclear bodies de novo at the periphery of the nucleolus when fused with Nuc2. (B) The nuclear bodies formed de novo at the periphery of the nucleolus contained components of photobodies. The nucleolus is labeled by Nuc2-mCherry. (C) Nucleated body by Nuc2-COP1-YFP at the periphery of the nucleolus did not colocalize with D-bodies or nuclear speckles marked by CFP-DCL1 or SCL33-CFP, respectively. (D) Quantitative analysis of efficiency of de novo photobody formation via tethering of different components to the nucleolus by Nuc2. Values represent averages (n = 110–200 cells) from three independent experiments. Bars, 5 μm.

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